A variant mitochondrial DNA arrangement specific toPetunia stable sterile somatic hybrids |
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Authors: | Maury L Boeshore Maureen R Hanson Shamay Izhar |
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Institution: | (1) Department of Biology, University of Virginia, 22901 Charlottesville, VA, U.S.A.;(2) Division of Plant Genetics and Breeding, Agriculture Research Organization, The Volcani Center, 52258 Bet Dagan, Israel;(3) Present address: Experimental Plant Genetics, The Upjohn Company, 49001 Kalamazoo, MI, U.S.A. |
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Abstract: | We have characterized two related regions of twoPetunia mitochondrial genomes in order to understand how plant mt genomes from a cytoplasmic male sterile (cms) line and a fertile
line diverge from one another. Restriction maps of these regions indicate that a sequence arrangement shared by the two genomes
adjoins sequences which are not shared at the corresponding locations in the two genomes. A point where the mt genomes from
the cms line and the fertile lines diverge from each other was identified and mapped.
Previously we had observed that somatic hybrids constructed from the cms and the fertile line contained mt genomes carrying
new combinations of parental mtDNA restriction fragments (3). Using the restriction maps of the two related mtDNA regions,
a mtDNA arrangement unique to the cms parent could be shown to be present in all 17 stable sterile somatic hybrids tested
and none of the 24 stable fertile somatic hybrids tested. This data does not exclude the possibility that additional, as yet
unidentified, mtDNA arrangements unique to the cms parent might also be found exclusively in sterile somatic hybrids. Whether
or not the sterile parental mtDNA arrangement reported here is functionally related to cms, it apparently segregates with
cms in somatic hybrids. |
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Keywords: | Petunia somatic hybrids mitochondrial DNA cytoplasmic male sterility mitochondrial genome divergence |
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