The requirement of Artemis in double-strand break repair depends on the type of DNA damage |
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Authors: | Kurosawa Aya Koyama Hideki Takayama Shinichi Miki Kensuke Ayusawa Dai Fujii Michihiko Iiizumi Susumu Adachi Noritaka |
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Institution: | Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan. |
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Abstract: | Artemis is a recently identified factor involved in V(D)J recombination and nonhomologous end joining (NHEJ) of DNA double-strand break (DSB) repair. Here, we performed targeted disruption of the Artemis gene (ARTEMIS) in the human pre-B cell line Nalm-6. Unexpectedly, we found that cells lacking Artemis exhibit increased sensitivity to low doses, but not high doses, of ionizing radiation. We also show that ARTEMIS-deficient cells are hypersensitive to the topoisomerase II inhibitor etoposide, but to a much lesser extent than cells lacking DNA ligase IV, a critical component of NHEJ. Unlike DNA ligase IV-deficient cells, ARTEMIS-deficient cells were not hypersensitive to ICRF-193, a topoisomerase II inhibitor that does not stabilize topoisomerase II-DNA cleavable complexes. Collectively, our results suggest that Artemis only partially participates in the NHEJ pathway to repair DSBs in human somatic cells. |
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