Neural crest cells: temperature-dependent transformation by Rous sarcoma virus. |
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Authors: | J H Greenberg J P Bader |
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Affiliation: | 1. Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health, Bethesda, MA 20014 U.S.A.;7. Laboratory of Tumor Virus Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MA 20014 U.S.A.;1. Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan;2. Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan;3. Atmospheric Environment and Applied Meteorology Research Department, Meteorological Research Institute, 1-1 Nagamine, Tsukuba, Ibaraki 305-0052, Japan;1. State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan 430072, China;2. Key Laboratory of Rock Mechanics in Hydraulic Structural Engineering, Ministry of Education, Wuhan University, Wuhan 430072, China;3. State Key Laboratory of Hydraulic Engineering Simulation and Safety, Tianjin University, Tianjin 300072, China;4. Nanchang University, Nanchang 330000, China;1. Department of Zoology, Faculty of Science, Charles University in Prague, Prague, Czech Republic;2. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA;3. South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic |
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Abstract: | Cranial neural crest cells from chick embryos, when cultured under appropriate conditions, differentiate after approx. 1 week into pigmented cells. Neurol crest cells were infested with a mutant (RSV-BH-Ta) of the Bryan 'high titer' strain of Rous sarcoma virus on the second day of culture before the cells were morphologically differentiated, or later after they became pigmented. Cells infected and maintained at the temperature permissive for transformation (37 degrees C) proliferated rapidly compared to uninfected cells are produced extensive cytoplasmic vacuoles in a fashion similar to other types of cells transformed with RSV-BH-Ta at 37 degrees C. Cells infected and maintained at the non-permissive temperature for transformation (41 degrees C) also proliferated rapidly but did not become morphologically transformed. Transformation occurred reversibly following a shift of temperature. Infection of morphologically undifferentiated neural crest cells at either temperature prevented their differentiation into pigment cells, and infection of pigmented neural crest cells at either temperature led to a gradual loss of pigmentation. These results suggest that even at the non-permissive temperature the virus may regulate the state of differentiation of certain types of cells. |
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