HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi |
| |
| Authors: | Nóbrega Otávio T Santana Jaime M Sturm Nancy R Teixeira Antônio R L Campbell David A |
| |
| Affiliation: | Laboratório Multidisciplinar de Pesquisa em Doen?a de Chagas, Faculdade de Medicina, Universidade de Brasília, Brasília, DF, Brazil. |
| |
| Abstract: | This work describes the development and functional testing of two episomes for stable transfection of Trypanosoma cruzi. pHygD contained the 5'- and 3'- flanking regions of the gene encoding the cathepsin B-like protease of T. cruzi as functional trans-splicing and polyadenylation signals for the hygR ORF. Evidence is presented to support extrachromosomal maintenance and organization as tandem repeats in transfected parasites. pPac was derived from pHygD by replacement of the entire hygR ORF with a purR coding region. The ability to modify pHygD and the availability of the complete DNA sequence make these plasmids useful tools for the genetic manipulation of T. cruzi. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
