Chromosome localization and gene synteny of the major histocompatibility complex in the owl monkey,Aotus |
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Authors: | Nancy S F Ma Therese Simeone Joanne McLean Peter Parham |
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Institution: | (1) New England Regional Primate Research Center, Harvard Medical School, 1 Pine Hill Drive, 01772 Southborough, Massachusetts;(2) Sidney Farber Cancer Institute, Harvard Medical School, 44 Binney Street, 02115 Boston, Massachusetts |
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Abstract: | Rodent cells were hybridized with owl monkey (Aotus) cells of karyotypes II, III, V, and VI. Aotus-rodent somatic hybrid lines preferentially segregating Aotus chromosomes were selected to determine the chromosomal location of the major histocompatibility complex and other genes with which it is syntenic in man. Based on correlation between concordant segregation of the chromosome as visualized by G-banding and expression of the Aotus antigens or enzymes in independent Aotus-rodent hybrid clones, we have assigned Aotus gene loci for the MHC, GLO, ME1, SOD2, and PGM3 to Aotus chromosome 9 of karyotype VI (2n=49/50), chromosome 10 of karyotype V (2n=46), and chromosome 7 of karyotypes II and III (2n = 54 and 53). On the basis of banding patterns we previously postulated that these chromosomes of the different karyotypes were homologous. The gene assignments reported here provide independent evidence for that hypothesis. Aotus chromosomes 9 (K-VI), 10 (K-V), and 7 (K-II, III) are homologous to human chromosome 6 in that they all code for the MHC, GLO, ME1, SOD2, and PGM3. The structural differences between these homologous chromosomes probably resulted from a pericentric inversion.Abbreviations used in this paper MHC
major histocompatibility complex
- HLA
human lymphocyte antigen
- PGM3
phosphoglucomutase-3
- ME1
cytoplasmic malic enzyme-1
- SOD2
superoxide dismutase-B
- GLO
glyoxalase 1
- OMLA
owl monkey leukocyte antigens
- K
karyotype
- 2-M
2-microglobulin
- DMEM
Dulbecco's modification of Eagle's medium
- PEG
polyethylene glycol
- HAT
hypoxanthine, aminopterin, and thymidine
- KC1
potassium chloride
- G-band-trypsin
Giemsa band |
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