Transient expression of hepatitis B virus surface antigen (HBsAg) gene in monkey cells by a SV40-based virus vector |
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| Authors: | Soraia A C Jorge Maria Concepcion de la Hera Angela M M Spina João Renato R Pinho Carlos F M Menck |
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| Institution: | (1) Depto. de Biologia, Instituto de Biociências, Universidade de São Paulo, CP 11461, 05422-970 São Paulo, SP, Brazil;(2) Instituto Adolfo Lutz, Av. Dr. Arnaldo, 335, 01246-902 São Paulo, SP, Brazil;(3) Present address: Depto, de Genética, Facultad de Ciências, Universidad de Cordoba, Cordoba, Spain;(4) Depto. de Biologia, Instituto de Biociências, Universidade de São Paulo, CP 11461, 05422-970 São Paulo, SP, Brazil |
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| Abstract: | Summary We have constructed a recombinant SV40-based vector carrying the S gene coding for the hepatitis B virus surface antigen (HBsAg). This vector replicates as an episome in monkey COS7 cells, producing high levels of hepatitis B virus surface antigen (HBsAg), which is liberated in the cell medium, probably as a membrane vesicule. The vector also carries the SV40-late genes and produces recombinant viruses. These viruses were used to infect fresh cell culture, with detection of HBsAg in the medium. Thus, this virus vector can efficiently transduce the gene for HBsAg. |
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