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Efficiently differentiating vascular endothelial cells from adipose tissue-derived mesenchymal stem cells in serum-free culture |
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| Authors: | Masamitsu Konno Tatsuo S Hamazaki Satsuki Fukuda Hideho Uchiyama Hitoshi Okochi Makoto Asashima |
| Institution: | a Department of Regenerative Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, Japan b Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113-8510, Japan c International Graduate School of Arts and Science, Yokohama City University, Yokohama 236-0027, Japan d Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan |
| Abstract: | Adipose tissue-derived mesenchymal stem cells (ASCs) have been reported to be multipotent and to differentiate into various cell types, including osteocytes, adipocytes, chondrocytes, and neural cells. Recently, many authors have reported that ASCs are also able to differentiate into vascular endothelial cells (VECs) in vitro. However, these reports included the use of medium containing fetal bovine serum for endothelial differentiation. In the present study, we have developed a novel method for differentiating mouse ASCs into VECs under serum-free conditions. After the differentiation culture, over 80% of the cells expressed vascular endothelial-specific marker proteins and could take up low-density lipoprotein in vitro. This protocol should be helpful in clarifying the mechanisms of ASC differentiation into the VSC lineage. |
| Keywords: | Adipose-derived mesenchymal stem cell Vascular endothelial cell Differentiation Serum-free culture FGF2 |
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