Antisense RNA based down-regulation of RNaseE in E.coli |
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Authors: | Christian Kemmer Peter Neubauer |
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Institution: | (1) Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, P. O. Box 4300, FIN-90014 Oulu, Finland |
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Abstract: | Background Messenger RNA decay is an important mechanism for controlling gene expression in all organisms. The rate of the mRNA degradation
directly affects the steady state concentration of mRNAs and therefore influences the protein synthesis. RNaseE has a key
importance for the general mRNA decay in E.coli. While RNaseE initiates the degradation of most mRNAs in E.coli, it is likely that the enzyme is also responsible for the degradation of recombinant RNAs. As RNaseE is essential for cell
viability and knockout mutants cannot be cultured, we investigated the possibility for a down-regulation of the intracellular
level of RNaseE by antisense RNAs. During this study, an antisense RNA based approach could be established which revealed
a strong reduction of the intracellular level of RNaseE in E.coli. |
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Keywords: | |
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