Increased susceptibility to tumor necrosis factor-α in butyric acid-induced apoptosis is caused by downregulation of cFLIP expression in Jurkat T cells |
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| Authors: | Shintaro Seto Tomoko Kurita-Ochiai Kuniyasu Ochiai |
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| Institution: | Division of Microbiology, Department of Oral Biology and Tissue Engineering, Meikai University School of Dentistry, Saitama;, Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba;, Department of Bacteriology, Nihon University School of Dentistry;, Divisions of Immunology and Pathology, Dental Research Center of Nihon University, Chiyoda, Tokyo, Japan |
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| Abstract: | Butyric acid is one of the major extracellular metabolites of periodontopathic Gram-negative bacteria. We previously demonstrated that butyric acid induced apoptosis in human T cells. In the present study, we examined the interaction between butyric acid and TNF-α in Jurkat T-cell apoptosis. Simultaneous treatment with TNF-α enhanced butyric acid-induced apoptosis by promoting caspase activity more than was achieved by either reagent alone. We examined which genes were associated with the increased susceptibility to TNF-α caused by butyric acid, and revealed that expression of cFLIP decreased with increased concentrations of butyric acid. Furthermore, exogenous expression of cFLIP protein suppressed the enhancing effect by TNF-α in the apoptosis. These results suggest that butyric acid downregulates cFLIP expression and increases the susceptibility to TNF-α by activating caspases via the death receptor signal. |
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| Keywords: | apoptosis butyric acid cellular FADD-like ICE-inhibitory protein tumor necrosis factor alpha |
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