High expression level of a gene coding for a chloroplastic amino acid selective channel protein is correlated to cold acclimation in cereals |
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Authors: | Baldi Paolo Grossi Maria Pecchioni Nicola Valè Giampiero Cattivelli Luigi |
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Institution: | (1) Istituto Sperimentale per la Cerealicoltura, Via S. Protaso 302, 29017 Fiorenzuola d'Arda (PC), Italy |
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Abstract: | A cold-regulated gene (cor tmc-ap3) coding for a putative chloroplastic amino acid selective channel protein was isolated from cold-treated barley leaves combining the differential display and the 5 -RACE techniques. Cor tmc-ap3 is expressed at low level under normal growing temperature, and its expression is strongly enhanced after cold treatment. A positive correlation between the expression of cor tmc-ap3 and frost tolerance was found both among barley cultivars and among cereal species. The COR TMC-AP3 protein was expressed in vitro, purified and used to raise a polyclonal antibody. Western analysis showed that the cor tmc-ap3 gene product is localized to the chloroplastic outer envelope fraction, supporting its putative function. The frost-resistant winter cultivar Onice accumulated COR TMC-AP3 more rapidly and at a higher level than the frost-susceptible spring cultivar Gitane. After 28 days of cold acclimation the winter cultivar had about 2-fold more protein than the spring genotype. All these results suggest that an increased amount of a chloroplastic amino acid selective channel protein could be required for cold acclimation in cereals. Hypotheses about the role of COR TMC-AP3 during the hardening process are discussed. |
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Keywords: | 5 -RACE" target="_blank">gif" alt="prime" align="BASELINE" BORDER="0">-RACE cold resistance cold-regulated gene differential display pore protein |
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