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Analysis of picogram quantities of indole-3-acetic acid by high performance liquid chromatography-fluorescence procedures |
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| Authors: | Alan Crozier Karen Loferski Joe B Zaerr Roy O Morris |
| Institution: | (1) Department of Forest Science, Oregon State University, 97331 Corvallis, OR, USA;(2) Department of Agricultural Chemistry, Oregon State University, 97331 Corvallis, OR, USA;(3) Present address: Botany Department, University, G12 8QQ Glasgow, Scotland |
| Abstract: | The use of spectrofluorimeter coupled to a reverse phase high performance liquid chromatography column permits selective detection of indole-3-acetic acid at the low picogram level. The value of the technique is demonstrated by the analysis of endogenous IAA in elongating shoots, xylem sap and callus of Douglas-fir. The data are also used to illustrate a procedure whereby the accuracy of chromatographic analyses can be verified within definable probability limits.Abbreviations GC-MS combined gas chromatography-mass spectrometry - HPLC high performance liquid chromatography - IAA indole-3-acetic acid - SEC steric exclusion chromatography - SICM selected ion current monitoring Technical Paper No. 5379 from the Oregon State University Agricultural Experiment Station |
| Keywords: | Auxin (analysis) High performance liquid chromatography Pseudotsuga Spectrofluorimetry |
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