三棱黄酮体外诱导A549及MCF-7细胞S/G2周期停滞的研究

为了研究三棱黄酮(Rhizoma Sparganii flavonoids,RSF)对雌激素受体阳性(estrogen receptor positive,ER+)恶性肿瘤细胞株A549与MCF-7的细胞毒作用和机制,本次试验制备含188、375、750μg/mL RSF的DMEM培养基(RSFD-188、RSFD-375、RSFD-750),与贴壁后的A549及MCF-7细胞共培养3 d,含1%甲醇的DMEM培养基为对照,使用MTT法、免疫荧光技术、流式细胞术比较组间细胞的增殖活性、形态(anti-a-tublin)、凋亡率以及细胞周期的差异。试验结果显示,RSF可呈剂量性抑制A549与MCF-7细胞的增殖活性、增加凋亡小体比率、诱导细胞骨架形态异常改变;与RSFD-375、RSFD-750共培养的A549与MCF-7细胞表现为明显的S/G2细胞周期停滞。所得实验结果表明,RSF对A549及MCF-7细胞具有明确的细胞毒理作用,其抑制细胞增殖活性的机制与诱导细胞的S/G2细胞周期停滞相关。

Flavonoids of Rhizoma Sparaganii Induce S/G2 Stage Arrest in A549 and MCF-7 Cells

To investigate the cytotoxicity effects and mechanisms on estrogen receptor positive(ER+) cancer cell lines A549 and MCF-7 of the Rhizoma Sparganii flavonoids(RSF),A549 or MCF-7 cells in DMEM mediums with 188,375,and 750 μg/mL RSF were cultured for 3 days,respectively.DMEM with 1% methanol culture cells as control.The cell proliferation activity,anti-a-tublin form,apoptosis as well as cell cycle differences between groups were studied by MTT,immunofluorescence,and flow cytometry technology.As a result,compare to the 1% methanol control groups,RSF could inhibit the proliferation activity,increase the rate of apoptotic bodies,and induce cytoskeleton disorder of the A549 and MCF-7 cells with the dose-related.In addition,S/G2 stage arrests were induced obviously in A549 and MCF-7 cells cultured with 375 and 750 μg/mL RSF.The results indicated that RSF showed a notable cytotoxicity against A549 and MCF-7 cells,and the inhibitions of cell proliferation were related with S/G2 cell cycle arrests.