Novel methods of genome shuffling in <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> |
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Authors: | Lihua Hou |
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Institution: | (1) Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China |
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Abstract: | Genome shuffling can improve complex phenotypes; however, there are several obstacles towards its broader applicability due
to increased complexity of eukaryotic cells. Here, we describe novel, efficient and reliable methods for genome shuffling
to increase ethanol production of Saccharomyces cerevisiae. Using yeast sexual and asexual reproduction by itself, mutant diploid cells were shuffled through highly efficient sporulation
and adequate cross among the haploid cells, followed by selection on the special plates. The selected strain obtained after
three round genome shuffling not only distinctly improved the resistance to ethanol, but also, increased ethanol yield by
up to 13% compared with the control. |
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Keywords: | Genome shuffling Novel methods Saccharomyces cerevisiae Ethanol |
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