An improved glucoseoxidase--peroxidase-coupled assay for -fructofuranosidase activity |
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| Authors: | O S Jorgensen B Andersen |
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| Institution: | Chemical Laboratory IV, H. C. Ørsted Institute, University of Copenhagen, Copenhagen, Denmark |
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| Abstract: | An improved glucoseoxidase-peroxidase-coupled assay for the determination of β-fructofuranosidase activity is described. The method makes use of the double effect of Tris (2-amino-2-hydroxymethylpropane-1,3-diol) as an inhibitor of both invertase and contaminating glucosidases. The method is very sensitive and is suitable for routine determinations. The total time needed for a single analysis is less than half an hour. |
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