High-throughput RNA extraction from plant samples based on homogenisation by reciprocal shaking in the presence of a mixture of sand and glass beads |
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Authors: | Kristel Eggermont Inge J Goderis Willem F Broekaert |
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Institution: | (1) F.A. Janssens Laboratorium voor Genetica, Katholieke Universiteit Leuven, Willem de Croylaan 42, B-3001 Heverlee, Belgium |
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Abstract: | We describe here a reliable high-throughput method for extraction of RNA from fresh or frozen plant tissue that obviates laborious
and time-consuming homogenisation by mortar and pestle. The method is based on homogenisation by high-speed reciprocal shaking
in presence of a mixture of inexpensive abrasive materials; i.e., quartz sand and glass beads. After homogenisation, the method
follows a standard procedure for RNA extraction by phenol/LiCl. Yield and quality of RNA obtained by homogenisation with the
sand/glass bead mix are identical to those obtained by mortar and pestle. |
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Keywords: | Arabidopsis thaliana glass beads reciprocal shaking RNA extraction sand |
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