Role of mitochondria in ethanol tolerance of Saccharomyces cerevisiae |
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Authors: | Andrés Aguilera Tahía Benítez |
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Institution: | (1) Departmento de Genética, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain;(2) Present address: Institut für Genetik, Technische Hochschule Darmstadt, D-6100 Darmstadt, Germany |
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Abstract: | The presence of active mitochondria and oxidative metabolism is shown to be essential to maintain low inhibition levels by ethanol of the growth rate (), fermentation rate (v) or respiration rate () of Saccharomyces cerevisiae wild type strain S288C. Cells which have respiratory metabolism show K
i (ethanol inhibition constant) values for , v and , higher (K
i>1 M) than those of petite mutants or grande strains grown in anaerobiosis (K
i=0.7 M). In addition, the relationship between or v and ethanol concentration is linear in cells with respiratory metabolism and exponential in cells lacking respiration. When functional mitochondria are transferred to petite mutants, the resulting strain shows K
i values similar to those of the grande strain and the inhibition of and v by increasing ethanol concentrations becomes linear. |
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Keywords: | Ethanol tolerance Cytoplasmic inheritance Mitochondria Growth rate Fermentation rate Ethanol inhibition constant Aerobiosis Anaerobiosis S cerevisiae |
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