The jury is out on "guilt by association" trials. |
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| Authors: | Jennifer I Semple Christopher M Sanderson R Duncan Campbell |
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| Affiliation: | A postdoctoral scientist at the MRC HGMP Resource Centre. Their research interests include functional analysis of genes in the MHC, protein-protein interaction mapping by yeast two hybrid, and the development of high throughput approaches to functional genomics |
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| Abstract: | ![]()
The availability of comprehensive protein-protein interaction maps will significantly enhance medical research and aid the functional characterisation of novel genes. To date, the largest scale studies of protein-protein interactions have used the yeast two hybrid method. In this review we take a closer look at the different approaches used in these studies and discuss some key considerations that should be taken into account when designing high throughput interaction mapping projects. |
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| Keywords: | yeast two hybrid protein-protein interactions functional genomics high throughput false positives false negatives |
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