Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection |
| |
Authors: | Loret S Nollevaux G Remacle R Klimek M Barakat I Deloyer P Grandfils C Dandrifosse G |
| |
Institution: | Department of Biochemistry and General Physiology, University of Liege, B-4000 Liege, Belgium. suzanne.loret@fundp.ac.be |
| |
Abstract: | We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20 +/- 9.30% and 0.12 +/- 0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52 +/- 0.35 expressing a low diffusion of this poorly permeability marker. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|