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| 初级代谢产物和有性生殖促进剂对番茄红素发酵的影响 | |
| 引用本文： | 王常玲,顾秋亚,余晓斌.初级代谢产物和有性生殖促进剂对番茄红素发酵的影响[J].生物加工过程,2009,7(6):40-44. |
| 作者姓名： | 王常玲 顾秋亚 余晓斌 |
| 作者单位： | 1. 江南大学,生物工程学院,无锡,214122;江南大学,工业生物技术教育部重点实验室,无锡,214122 2. 江南大学,生物工程学院,无锡,214122 3. 江南大学,工业生物技术教育部重点实验室,无锡,214122 |
| 基金项目： | 江南大学青年科学基金资助项目，教育部长江学者与创新团队发展计划资助项目 |
| 摘 要： | 考察几种初级代谢中间产物对三孢布拉霉发酵生产番茄红素的影响，以及卵磷脂对三孢布拉霉正负菌接合孢子及发酵的影响。结果表明：发酵24h分别添加2．0％的柠檬酸和2．0％的三孢酸，番茄红素的产量分别达到0．99g／L和1．26g/L，比对照分别提高39．43％和32．63％；卵磷脂的添加能促进三孢布拉霉两性接合孢子的形成，进而促进番茄红素的合成，当大豆卵磷脂的添加量为0．3％时，番茄红素的产量为1．58g/L，比对照提高56．44％。 |
| 关 键 词： | 三孢布拉霉 初级代谢物 有性生殖 番茄红素 大豆卵磷脂 |
Effects of primary metabolites and promoters of sexual reproduction on lycopene biosynthesis with Blakeslea trispora |
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| WANG Chang-ling,GU Qiu-ya,YU Xiao-bin.Effects of primary metabolites and promoters of sexual reproduction on lycopene biosynthesis with Blakeslea trispora[J].Chinese Journal of Bioprocess Engineering,2009,7(6):40-44. | |
| Authors: | WANG Chang-ling GU Qiu-ya YU Xiao-bin |
| Institution: | 1. College of Biotechnology, Jiangnan University, Wuxi 214122, China; 2. Key Laboratory of Industrial Biotechnology of the Ministry of Education, Jiangnan University, Wuxi 214122, China) |
| Abstract: | Effects of primary metabolites and promoters of zygote formation on the lycopene biosynthesis with Blakeslea trispora were investigated. Results showed that the citric acid and the trisporic acid improved the lycopene production. When 2.0% citric acid and 2.0% trisporic acid were added to the culture, respectively, the lycopene production was 0.99% g/L and 1.26 g/L, it was 39.43% and 32.63% higher than the process without addition of soy lecithin. Results indicated that the soy lecithin promoted the carotenoid production, when 0.3% soy lecithin were added to the culture of Blakeska trispora, the lycopene production was 1.58 g/L. It was 56.44% higher than the process without addition of soy lecithin. |
| Keywords: | Blakeslea trispora primary metabolites sexual reproduction lycopene soy lecithin |
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