一种与受精有关的人精子膜蛋白（BS－17）的分离纯化

本文用盐分级分离，ＭｏｎｏＱＦＰＬＣ及ＳＤＳ－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）等方法从人精子ＣＨＡＰＳ抽提液中分离纯化出一种与不育病人血清中抗精子抗体发生特异反应的ＢＳ－１７人精子膜蛋白。该蛋白为一糖蛋白，分子量为１７．５５±２．１５ｋＤ，等电点为５．６５，中性己糖含量为１６．６７％。在人精子上主要分布于顶体区域，不同于已有报导的人精子膜蛋白。在体外实验中抗ＢＳ－１７多克隆血清可以显著影响人精子的获能（ｐ＜０．０２５）和对去透明带仓鼠卵的穿透（ｐ＜０．００５），但不影响人精子运动性及与去透明酯酸带仓鼠卵的结合。小鼠体内被动免疫实验结果证明抗ＢＳ－１７多抗血清具有明显地抑制受精的功能（ｐ＜０．００１）。

Purification of a Human Sperm Protein (BS-17) Related to Fertilization

Serum obtained from an infertile subject possessed antibodies that interacted with a human sperm glycoprotein with an estimated Mr of 17 550 and pI of 5. 65 containing 17.67% neutral hexose and designated as the BS-17 component. Polyclonal antibodies raised against the BS-17 antigen blocked the capacity of human sperm to fertilize zona-free hamster ova in vitro; however, the antibodies did not influence the binding of human sperm to zonafree ova or alter the motility of human sperm. The antibodies inhibited the capacity of mouse sperm to fertilize ova upon in vivo insemination. The BS-17 antigen was detected in human,rat, mouse, rabbit and hamster sperm by an immunocytochemical method, using polyclonal anti-BS-17 antibodies. Intense staining occurred over the surface of the acrosomal region of all mammalian sperm.