Isolation of <Emphasis Type="BoldItalic">Serratia marcescens</Emphasis> as a chondroitinase-producing bacterium and purification of a novel chondroitinase AC |
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Authors: | Tao?Ke Long?Zhangfu Gao?Qing Tao?Yong Jin?Hong Ran?Hongyan Liu?Kun Email author" target="_blank">Liu? ShiguiEmail author |
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Institution: | (1) National Laboratory of Grassland Biocontrol Engineering, Sichuan University, Chengdu, 610064, China;(2) Institute of Chuangxin Biotechnology, Sichuan University, Chengdu, 610064, China |
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Abstract: | A strain of Serratia marcescens that produced chondroitinase was isolated from soil. It produced a novel chondroitinase AC, which was purified to homogeneity. The enzyme was composed of two identical subunits of 35 kDa as revealed by SDS-PAGE and gel filtration. The isoelectric point for the chondroitinase AC was 7.19. Its optimal activity was at pH 7.5 and 40 °C. The purified enzyme was active on chondroitin sulfates A and C and hyaluronic acid, but was not with chondroitin sulfate B (dermatan sulfate), heparin or heparan sulfate. The apparent Km and Vmax of the chondroitinase AC for chondroitin sulfate A were 0.4 mg ml–1 and 85 mmol min–1 mg–1, respectively, and for chondroitin sulfate C, 0.5 mg ml–1 and 103 mmol min–1 mg–1, respectively. |
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Keywords: | chondroitinase AC identification purification Serratia marcescens |
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