Methodology for using a universal primer to label amplified DNA segments for molecular analysis |
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Authors: | Dong-chuan Guo Dianna M Milewicz |
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Institution: | Department of Internal Medicine, University of Texas - Houston Medical School, MSB 1.410, 6431 Fannin, Houston, TX 77030, USA. |
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Abstract: | Detection of human DNA polymorphisms using high throughput methods often relies on generating a labeled DNA fraament which is generated by PCR using sequence-specific primers with an end labeled tag to detect a variant. The disadvantage of the synthesis of an end-labeled, sequence-specific primer to assay each DNA variant lies in the costs and time consume. In this report, we have demonstrated a methodology that can generate labeled DNA fragments using a labeled universal primer instead of requiring sequence-specific primers for each DNA variant. |
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Keywords: | biotin-labeled primer fluorescently labeled primer genotyping Pyrosequencing universal primer |
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