Molecular properties of pyrophosphate:fructose-6-phosphate phosphotransferase from potato tuber |
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| Authors: | N J Kruger D T Dennis |
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| Affiliation: | 1. School of Automation, Hangzhou Dianzi University, Hanzhou, Zhejiang Province 310018, China;2. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang Province 310058, China;3. Jinhua Academy of Agricultural Sciences, Jinhua, Zhejiang Province 321017, China;1. Plant Molecular Genetics Department, Centro Nacional de Biotecnología/CSIC, Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain;2. Genomics Unit, Centro Nacional de Biotecnología/CSIC, Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain;1. Key Laboratory of Natural Medicine and Immune-Engineering of Henan Province, Henan University, Kaifeng, Henan Province 475004, China;2. Shangqiu Medical College, Shangqiu, Henan Province 476100, China;3. Bureau of Commodity Quality Supervision and Inspection, Hengyang, Hunan Province 421001, China;4. School of Public Health, Zhengzhou University, Zhengzhou, Henan Province 450001, China;1. Institute of Integrative Biology, ETH Zurich & Zurich–Basel Plant Science Center, Universitätstrasse 16, 8092 Zurich, Switzerland;2. Department of Plant and Microbial Biology, University of Zurich and Zurich–Basel Plant Science Center, Zollikerstrasse 107, 8008 Zurich, Switzerland;3. GAFL INRAE, Allée des Chênes 67, 84140 Montfavet, France |
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| Abstract: | ![]()
Pyrophosphate:fructose-6-phosphate phosphotransferase (PFP) from potato tubers has been purified to homogeneity. The enzyme contains two polypeptides with apparent relative molecular mass (Mr) values of 65,000 and 60,000. These polypeptides give different peptide fragments after limited proteolytic digestion. Antibodies raised against each polypeptide separately are specific for that polypeptide, but both antisera are capable of immunoprecipitating native PFP activity. These antibodies also recognize similar pairs of polypeptides in a range of other plant tissues that contain PFP activity. Based on gel filtration, the Mr value of potato tuber PFP is 265,000. This suggests that the enzyme is a heterotetramer composed of two polypeptides with Mr values of 65,000 and 60,000. In the presence of pyrophosphate, potato PFP dissociates into a 130,000 dimer. |
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