Effect of solvents on the catalytic activity of firefly luciferase |
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| Authors: | Larry J. Kricka Marlene De Luca |
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| Affiliation: | Department of Chemistry, University of California, San Diego, La Jolla, California 92093 U.S.A. |
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| Abstract: | Various solvents stimulate the catalytic activity of firefly luciferase, up to sevenfold. Polyvinylpyrrolidone, polyethylene glycols, and nonionic detergents such as Triton X-100 were the most effective stimulators of the enzyme. Both peak light and total light emission were enhanced in the presence of these solvents indicating an increased turnover of the enzyme. The primary effect of the solvents is on the oxidative reaction rather than the activation reaction. All the experimental data support the hypothesis that the presence of solvent promotes the dissociation of the inhibitory product from the enzyme. |
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| Keywords: | Author to whom correspondence should be addressed: Chemistry Department M-001 University of California San Diego La Jolla Calif. 92093. |
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