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| 2011—2016年上海地区手足口病病原谱及柯萨奇病毒A组6型不同基因型感染细胞的差异研究 | |
| 引用本文： | 张晓玲,俞慧菊,刘祎,代发辉,宋志刚,王蔚. 2011—2016年上海地区手足口病病原谱及柯萨奇病毒A组6型不同基因型感染细胞的差异研究[J]. 微生物与感染, 2018, 13(2): 84-89. DOI: 10.3969/j.issn.1673-6184.2018.02.004 |
| 作者姓名： | 张晓玲 俞慧菊 刘祎 代发辉 宋志刚 王蔚 |
| 作者单位： | 1. 复旦大学附属公共卫生临床中心，上海 201508; 2. 上海交通大学医学院附属新华医院，上海 200092; 3. 上海市公共卫生临床中心，上海 201508 |
| 基金项目： | “十二五”国家科技重大专项(2012ZX10004-211)，上海市公共卫生临床中心萌芽计划(2015MY01)，上海市科委2014年度“科技创新行动计划”生物医学领域产学研医合作项目(14DZ1940400、14DZ1940402)，上海市卫生和计划生育委员会科研课题(20134184) |
| 摘 要： | 本文旨在分析2011—2016年上海地区手足口病病原谱的构成和主要病原体流行规律，为手足口病的疫情防控和重症预警提供参考。采集2011—2016年哨点医院临床诊断为手足口病的患儿标本，首先进行肠道病毒通用型核酸和分型检测，其次对2012—2016年核酸检测为柯萨奇病毒A组6型(coxsackievirus A6，CA6)阳性样本进行基因型重组检测，分离重组型和非重组型CA6株，比较两者生物学特性。结果显示，2011—2012年秋季上海地区手足口病主要致病原为肠道病毒71型(enterovirus type 71，EV71)和CA16；2012年秋季起CA6成为主要病原体；2013—2014年重组型CA6(recombinant CA6，R CA6)占一定比例；2015—2016年非重组型CA6(non-recombinant CA6，NR CA6)成为优势流行株。R CA6和NR CA6代表株在RD细胞中增殖良好，在KB、MRC-5和HEp-2细胞中未见明显增殖。比较R CA6与NR CA6代表株接种于RD细胞后细胞活性下降的动态变化，未见显著差异。本研究证实，CA6为近年来上海地区手足口病的主要病原体，R CA6和NR CA6的构成比逐年变化，提示持续监测手足口病主要病原体并了解其生物学特性，对上海地区手足口病的防控预警及疫苗研发具有重要意义。 |
| 关 键 词： | 手足口病 肠道病毒71型 柯萨奇病毒A组6型 重组 |
Pathogen spectrums and characterization of hand,foot and mouth disease from 2011 to 2016 in Shanghai |
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| ZHANG Xiaoling,YU Huiju,LIU Yi,DAI Fahui,SONG Zhigang,WANG Wei. Pathogen spectrums and characterization of hand,foot and mouth disease from 2011 to 2016 in Shanghai[J]. Journal of Microbes and Infection, 2018, 13(2): 84-89. DOI: 10.3969/j.issn.1673-6184.2018.02.004 | |
| Authors: | ZHANG Xiaoling YU Huiju LIU Yi DAI Fahui SONG Zhigang WANG Wei |
| Affiliation: | 1. Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China; 2. Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3. Shanghai Public Health Clinical Center, Shanghai 201508, China |
| Abstract: | The present paper aims to analyze the pathogen spectrum of hand, foot and mouth disease (HFMD) and epidemic dynamics of main pathogens in Shanghai from 2011 to 2016. The samples were collected from children diagnosed with HFMD in sentinel hospitals from 2011 to 2016. General and classified tests for enteroviruses were carried out, and the biological characteristics of both recombined and non-recombined coxsackievirus A6 (CA6) strains were analyzed. It was found that the main pathogens of HFMD were enterovirus type 71 (EV71) and CA16 from 2011 to 2012 in Shanghai area, and CA6 became the main pathogen in the autumn of 2012. From 2013 to 2014, recombined CA6 (R CA6) occupied a certain proportion, but non-recombined CA6 (NR CA6) became dominant during 2015—2016. R CA6 and NR CA6 were highly proliferative in human rhabdomyoma cells (RD cells) but had no obvious proliferation in human oral epidermoid carcinoma cells (KB cells), human embryo lung cells (MRC-5 cells) and human laryngeal epidermoid carcinoma cells (HEp-2 cells). The results confirmed that CA6 was the major causative agent of HFMD in Shanghai in recent years. Both R CA6 and NR CA6 strains could infect RD cells. The detection ratio of R CA6 and NR CA6 changed year by year. These results indicate that monitoring and characterizing the main pathogens of HFMD continuously was a critical step for the prevention of HFMD and vaccine development. |
| Keywords: | Hand foot and mouth disease Enterovirus 71 Coxsackievirus A6 Recombination |
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