| 杜仲内生真菌的分离鉴定及抗菌活性研究 |
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| 引用本文： | 杨明琰,田 稼,马 瑜,孙 超,黄继红.杜仲内生真菌的分离鉴定及抗菌活性研究[J].西北植物学报,2012,32(1):193-198. |
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| 作者姓名： | 杨明琰 田 稼 马 瑜 孙 超 黄继红 |
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| 作者单位： | 1. 长安大学环境科学与工程学院,西安,710061
2. 陕西省微生物研究所,西安,710043 |
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| 基金项目： | 陕西省科学技术研究发展计划项目，陕西省科学院青年基金资助项目 |
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| 摘 要： | 采用组织分离法从秦岭药用植物杜仲茎部共分离到内生真菌38株,经形态学鉴定分属于9属,其中青霉属为杜仲内生真菌中的优势菌群,拟青霉属、交链孢属和无孢群属为常见属。对分离得到的杜仲内生真菌发酵液进行了抑菌实验,其中61%的菌株对测试细菌具有抗菌活性,表明杜仲内生真菌的抗菌活性十分普遍;34%的菌株对烟草赤星病菌、苹果炭疽病菌及辣椒炭疽病菌等多种植物病原菌具有较强的抑制活性,表明杜仲内生真菌在植物病害的生物防治领域具有进一步开发的价值。
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| 关 键 词： | 杜仲 内生真菌 分离 分类鉴定 抗菌活性 |
Isolation,Identification and Antimicrobial Activity of Endophyte Fungi from Medicinal Plant Eucommia ulmoides Oliv.in Qinling Mountain |
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| YANG Ming-yan,TIAN Ji,MA Yu,SUN Chao,HUANG Ji-hong.Isolation,Identification and Antimicrobial Activity of Endophyte Fungi from Medicinal Plant Eucommia ulmoides Oliv.in Qinling Mountain[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(1):193-198. |
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| Authors: | YANG Ming-yan TIAN Ji MA Yu SUN Chao HUANG Ji-hong |
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| Institution: | 1 School of Environmental Science and Engineering,Chang’an University,Xi’an 710061,China;2 Shaanxi Province Institute of Microbiology,Xi’an 710043,China) |
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| Abstract: | 38 strains of endophyte fungi belong to 9 genera were isolated from the stem of medicinal plant Eucommia ulmoides Oliv.collected in Qinling Mountain by the methods of tissue separation.Penicillium was the dominant group and Paecilomyces,Alternaria,mycelia sterilia were the common groups.The result of antimicrobial activity assay showed 61% endophyte fungi presented antibacterial activity,34% endophyte fungi presented antifungals activity towards Algernaria alternata,Colletotrichum gloeosporioides and C.capsici.It indicated antimicrobial activity was common in endophyte fungi isolated from E.ulmoides Oliv.and worthing for further studying in biological control of plant diseases. |
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| Keywords: | Eucommia ulmoides Oliv endophyte fungi isolation identification antimicrobial activity |
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