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A simple molecular approach to distinguish between two Arctic gadoid fishes Arctogadus glacialis (Peters, 1874) and Boreogadus saida (Lepechin, 1774)
Authors:Matias L. Madsen  Svein-Erik Fevolden  Jørgen S. Christiansen
Affiliation:1.Norwegian College of Fishery Science,University of Troms?,Troms?,Norway
Abstract:Correct phenotypical identification of and discrimination between Boreogadus saida and Arctogadus glacialis is challenging, especially for larvae and young fish. We propose the use of a single microsatellite genetic marker, Gmo8, to distinguish between the two gadoid fish species. Amplified allele frequencies differed considerably, with B. saida (n = 97) being almost exclusively monomorphic at this locus, whereas A. glacialis (n = 136) is highly polymorphic. There was a clear separation between the amplified allele ranges for the two species. The species specific properties of Gmo8 enables the use of this marker to distinguish between B. saida and A. glacialis.
Keywords:Arctogadus glacialis    Boreogadus saida   Species determination  Microsatellite marker
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