Structure of the major neutral oligosaccharide-alditols released from the egg jelly coats ofAxolotl maculatum. Characterization of the carbohydrate sequence GalNAc({eta}l-4)[Fuc({alpha}l-3)] GlcNAc({eta}l-3 6) |
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Authors: | Strecker Grard; Michel Jean; Wleruszeski ; Dominique Marie; Fontaine ; Plancke Yves |
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Institution: | Laboraloirc de Chiraie Biologique et Unit Mixte de Recherches du Centre National dc la Recherche Scientifique 111, Universit des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq Cedex, France |
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Abstract: | Several O-linked oligosaccharides of the jelly coat surroundingthe eggs of Axolotl maculatum were analysed by 1H-NMR spectroscopy.The four major oligosaccharide-alditols released by reductive-elimination display either the Lewisx(Lex) determinant or thesequence GalNAc(l-4)Fuc(l-3)]GlcNAc This last structure haspreviously been characterized in allergenically active oligosaccharidesisolated from the sea squirt H-antigen, and hi the N-linkedglycans of Schistosoma mansoni and human urokinase. It representsthe major carbohydrate chain found in A.macu-latum,the oviductof which constitutes an excellent source of l-4-acetylgalactosaminyltransferase activity. Moreover, the carbohydrate chains isolatedfrom A.maculatum are quite different from those found hi sevenother amphibian species, in which the presence of species-specificmaterial has been characterized. The role of carbohydrates appearsmore and more apparent during the fertilization process, andthe diversity of the O-linked oligosaccharides supports sucha biological role. amphibian egg jelly coats Axolotl maculatum/Hnmr/oligosccharide structure |
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