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RNA degradation compromises the reliability of microRNA expression profiling |
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| Authors: | David Ibberson Vladimir Benes Martina U Muckenthaler Mirco Castoldi |
| Affiliation: | 1. Genomics Core Facility, EMBL, Meyerhofstra?e 1, D-69117, Heidelberg, Germany 2. Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Im Neuenheimer Feld 156, D-69120, Heidelberg, Germany 3. Molecular Medicine Partnership Unit, Im Neuenheimer Feld 156, D-69120, Heidelberg, Germany |
| Abstract: | BackgroundMicroRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression and their expression is frequently altered in human diseases, including cancer. To correlate clinically relevant parameters with microRNA expression, total RNA is frequently prepared from samples that were archived for various time periods in frozen tissue banks but, unfortunately, RNA integrity is not always preserved in these frozen tissues. Here, we investigate whether experimentally induced RNA degradation affects microRNA expression profiles. |
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