Alkaloid patterns in Leucojum aestivum shoot culture cultivated at temporary immersion conditions |
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Authors: | Ivanov Ivan Georgiev Vasil Berkov Strahil Pavlov Atanas |
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Institution: | a Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000 Plovdiv, Bulgaria b AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164 Sofia, Bulgaria c University of Food Technologies, Department of Organic Chemistry and Microbiology, 26 Maritza Blvd, 4002 Plovdiv, Bulgaria |
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Abstract: | The alkaloid patterns in Leucojum aestivum L. shoot culture cultivated at temporary immersion conditions were investigated using gas chromatography-mass spectrometry. 18 alkaloids were identified, and galanthamine, hamayne and lycorine were dominant. The L. aestivum 80 shoot culture, cultivated at temporary immersion conditions, is a prospective biological matrix for obtaining wide range Amaryllidaceae alkaloids, showing valuable biological and pharmacological activities. The temperature of cultivation influenced enzyme activities, catalyzing phenol oxidative coupling of 4′-O-methylnorbelladine and formation of the different groups Amaryllidaceae alkaloids. Decreasing the temperature of cultivation of L. aestivum 80 shoot culture led to activation of para-ortho’ phenol oxidative coupling (formation of galanthamine type alkaloids) and inhibited ortho-para’ and para-para’ phenol oxidative coupling (formation of lycorine and haemanthamine types alkaloids). |
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Keywords: | BAP 6-benzylaminopurine GC/MS gas chromatography-mass spectrometry MS Murashige and Skoog nutrient medium NAA 1-naphthylacetic acid TIC total ion current |
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