Oligodendroglial and pan‐neural crest expression of Cre recombinase directed by Sox10 enhancer |
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| Authors: | Zachary E Stine Jimmy L Huynh Stacie K Loftus David U Gorkin Amirali H Salmasi Thomas Novak Todd Purves Ronald A Miller Anthony Antonellis John P Gearhart William J Pavan Andrew S McCallion |
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| Institution: | 1. McKusick‐Nathans Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland;2. Zachary E. Stine and Jimmy L. Huynh contributed equally to this work.;3. Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland;4. Department of Pediatric Urology, The Johns Hopkins University School of Medicine, Baltimore, Maryland;5. Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland;6. Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland |
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| Abstract: | Utilizing a recently identified Sox10 distal enhancer directing Cre expression, we report S4F:Cre, a transgenic mouse line capable of inducing recombination in oligodendroglia and all examined neural crest derived tissues. Assayed using R26R:LacZ reporter mice expression was detected in neural crest derived tissues including the forming facial skeleton, dorsal root ganglia, sympathetic ganglia, enteric nervous system, aortae, and melanoblasts, consistent with Sox10 expression. LacZ reporter expression was also detected in non‐neural crest derived tissues including the oligodendrocytes and the ventral neural tube. This line provides appreciable differences in Cre expression pattern from other transgenic mouse lines that mark neural crest populations, including additional populations defined by the expression of other SoxE proteins. The S4F:Cre transgenic line will thus serve as a powerful tool for lineage tracing, gene function characterization, and genome manipulation in these populations. genesis 47:765–770, 2009. © 2009 Wiley‐Liss, Inc. |
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| Keywords: | transgenic mouse Cre recombinase Sox10 oligodendroglia neural crest |
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