Respiratory activity of alginate-encapsulated Pseudomonas fluorescens cells introduced into soil |
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Authors: | Jack T Trevors |
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Institution: | (1) Department of Environmental Biology, University of Guelph, N1G 2W1 Guelph, Ontario, Canada |
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Abstract: | Summary Alginate-entrapped cells of Pseudomonas fluorescens were introduced into soil microcosms to evaluate their respiratory activity (O2 consumption and CO2 evolution) and survival during a 14-day incubation period at 20°C. Alginate-entrapped cells and cells resuspended in sterile distilled water and introduced into sterile soil exhibited relatively similar O2 consumption/CO2 evolution and survival over the 14-day period. The same treatments in non-sterile soil exhibited lower respiratory activity and a population density decrease of about 2.0 Log. cfu/g after 14 days. Alginate-entrapped bacterial cells may be a useful method for introducing genetically-engineered and non-engineered bacterial strains into the soil environment. |
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