Establishment of a novel testicular cell line from sterlet Acipenser ruthenus and evaluation of its applications |
| |
| Authors: | Ai Sun Hua Zhu Ying Dong Wei Wang Hong Xia Hu |
| |
| Institution: | Beijing Fisheries Research Institute & National Freshwater Fisheries Engineering Technology Research Center, Ministry of Science and Technology of China, Lab of Biological Technology and Breeding, Beijing Key Laboratory of Fishery Biotechnology, Beijing, China |
| |
| Abstract: | In this study, a cell line, designated as Acipenser ruthenus testis (ART), was successfully established from testis tissues of the sterlet Acipenser ruthenus and characterized by studying and comparing the expression of specific genes between the cell line and the parent gonad tissues. The results suggested that the developed ART cell line was composed of a mixture of germ cells and somatic cells. Ploidy analysis indicated that the cell line exhibited a high degree of genetic stability and that the cells remained in a good proliferating state after being subcultured to passage 80. |
| |
| Keywords: | Acipenser ruthenus cell line gene expression sturgeon testis transfection |
|
|
