紫芝细胞壁大分子量葡聚糖的纯化及结构鉴定

采用超微粉碎、热水浸提法从紫芝子实体水提残渣中获得细胞壁粗多糖,通过30%乙醇沉淀、20%醇洗的方法纯化出大分子量均一多糖GSCW30E-20E。苯酚硫酸法检测其多糖含量为98.03%,单糖组成分析显示其仅由葡萄糖组成,高效凝胶尺寸排阻色谱-多角度激光散射仪-示差折光检测技术测定其重均分子量为1.552×10 ⁶g/mol。通过红外光谱、甲基化及核磁共振分析对其结构进行解析,结果表明,GSCW30E-20E是一种β-D-葡聚糖,该多糖主链由β-(1,3)-糖苷键连接而成,每3个糖残基主链上通过β-(1,6)-糖苷键连有一个葡萄糖残基为支链。

Purification and structure elucidation of high molecular weight polysaccharide from the cell wall of Ganoderma sinense

Cell wall polysaccharides were extracted by hot water extraction of superfine comminuted powder from water-extracted residue of Ganoderma sinense fruiting bodies. A high molecular weight polysaccharide GSCW30E-20E was purified by ethanol precipitation. The total sugar content of GSCW30E-20E determined by phenol-sulfate method was 98.03%. HPAEC results showed that its monosaccharide component is only glucose. The average molecular weight of GSCW30E-20E determined by HPSEC-MALLS-RI system was 1.552×10 ⁶g/mol. Structure analysis by using infrared spectroscopy, methylation and NMR indicated that GSCW30E-20E was a beta-(1,3)-glucan linked to a glucose residue by a beta-(1,6)-glycoside bond. The degree of branching is 0.33.