金针菇交配型B第三个亚位点的发现与遗传

本研究在分析金针菇减数分裂重组热点时发现B交配型位点可能存在第三个亚位点,为此对同核菌株‘6-3’和‘6-21’的B交配型位点进行全基因组注释,获得8对同源信息素受体基因（pheromone receptor gene,pr）和3对同源信息素前体基因（pheromone precursor gene,pp）,组成了2个结构完整、相距326kb的亚位点α和β;‘6-21’菌株还发现1个‘6-3’菌株不具有的pr,组成第3个亚位点γ,它与亚位点α和β没有连锁。‘6-3’和‘6-21’配对形成异核体后出菇,分离获得142个单孢菌株,其B交配型有8种基因型,通过亲和性试验得到证实,同时还验证了第3个亚位点γ具有与α、β相同的功能。

The discovery and inheritance of the third mat-B sublocus in Flammulina filiformis

During analysis of the meiotic recombination hotspots, we speculated that there might be a third mat-B sublocus presenting in the genome of Flammulina filiformis. The genomes of the homokaryotic strains ‘6-3’ and ‘6-21’ were annotated, and 8 paired homologous pheromone receptor genes and 3 paired homologous pheromone precursor genes were found, which constituted two mat-B subloci (α and β) those were 326kb apart. An extra pheromone precursor gene was found in the strain ‘6-21’, which was absent in ‘6-3’, constituting the third mat-B sublocus γ unlinked with α and β. Heterocaryon formed by mating ‘6-3’ with ‘6-21’ was cultivated for fruiting, and 142 single spore strains were isolated from the fruit-bodies. As a result, there were 8 genotypes of mat-B confirmed by compatibility tests. The third sublocus of mat-B was verified to have the same function as α and β loci.