A Rhizobium tropici DNA region carrying the amino-terminal half of a nodD gene and a nod-box-like sequence confers host-range extension |
| |
| Authors: | Carolina Sousa Jorge Luis Folch Purificación Boloix Manuel Megías Noreide Nava Carmen Quinto |
| |
| Institution: | Departamento de Microbiología y Parasitologia, Universidad de Sevilla, Apartado Postal 874, 41003 Sevilla, Spain.;Departamento de Blología Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México. |
| |
| Abstract: | Rhizobium tropici CIAT899 is a broad-host-range strain that, in addition to Phaseolus, nodulates other plant legumes such as Leucaena and Macroptilium. The narrow-host-range of Rhizobium leguminosarum biovars phaseoli (strain CE3) and trifolii (strain RS1051) can be extended to Leucaena esculents and Phaseolus vulgaris plants, respectively, by the introduction of a DNA fragment 521 bp long, which carries 128 amino acids of the amino-terminal region of a nodD gene from R. tropici, as well as a putative nod-box-like sequence, divergently oriented. The 521 bp fragment, in the presence of L. esculenta or P. vulgaris root exudates, induced a R. leguminosarum bv. viciae nodA-lacZ fusion in either a CE3 or RS1051 background, respectively. |
| |
| Keywords: | |
|
|
