| 基因乙肝表面抗原纯化工艺的初步改进 |
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| 引用本文： | 周国林,郑菊酶.基因乙肝表面抗原纯化工艺的初步改进[J].微生物学免疫学进展,1994,22(3):13-16. |
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| 作者姓名： | 周国林 郑菊酶 |
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| 作者单位： | 卫生部兰州生物制品研究所 |
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| 摘 要： | 本文研究了新的层析介质（Ｃｅｌｌｕｆｉｎｅ）对原纯化工艺中ＳｅｐｈａｒｓｅＣＬ－４Ｂ柱层析纯化后的乙肝表面抗原（ＨＢｓＡｇ）组分及ＤＮＡ组分的进一步纯化效果。结果表明：该层析介质可以提高ＨＢｓＡｇ的纯度和收量，并对初步提纯ＤＮＡ组分中的乙肝表面抗原有一定意义。并首次发现ＤＮＡ组分中乙肝表面抗原的ＳＤＳ－ＰＡＧＢ图谱较正常基因乙肝表面抗原的图谱缺少３０ＫＤ的条带。
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| 关 键 词： | 重组乙肝表面抗原，DNA，柱层析 |
lmprovement in Purification of Recombinant HBsAg |
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| Zhou Guolin, Zheng Jumei, Zhang Ying.lmprovement in Purification of Recombinant HBsAg[J].Progress In Microbiology and Immunology,1994,22(3):13-16. |
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| Authors: | Zhou Guolin Zheng Jumei Zhang Ying |
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| Abstract: | It was studied that the effect of Cellufine Sulfate Media to purify the HBsAg components and DNA components obtained after the purification of sepharose CL-4B Results showed that this Media could increase the purity and recovery amount of recombinant HBsAg,and this Media could purify recombinant HBsAg in DNA components Preliminarily. It was the first time that We discovered that there was no 30KD band in SDS-PAGE of recombinant HBsAg in DNA components in comiparison with SDS-PAGE of standard recombinant HBsAg. |
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| Keywords: | rHBsAg DNA Column Chromatography |
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