枸杞多糖对肾性高血压大鼠离体血管反应性的影响及其机制

本工作利用两肾一夹肾性高血压大鼠模型,观察枸杞多糖对高血压大鼠血压的影响以及离体主动脉环丙皮细胞在调节血管张力中的功能改变,探讨ＬＢＰ对高血压发生发展的影响及其机制。结果表明,ＬＢＰ可防止２Ｋ１Ｃ大鼠高血压的形成。离体主动脉环灌流表明ＲＨ组对苯肾上腺素的收缩反应明显高于对照组,去除内皮后组间差异消失．

The effect of lycium barbarum polysaccharide on vascular tension in two-kidney, one clip model of hypertension

In the present study, the effects of lycium harbarum polysaccharide (LBP) on endothelial function in the twO-kidney, one clip model of hypertension were abserved.The results shawed that the increase of blood pressure in hypertension rats (HR) could be prevented significantly by treatment with 10% LBP. In isolated aortic rings of LBPtreated rats, the contraction of phenylepdrine (PE) was reduced as compared with HRrats. Removal of the endothelium abolished the difference of PE-induced vasoconstriction among groups. In vitro incubation of aortic rings from LBP-treated rats with methyl blue (MB) or N-nitro-harginine methyl ester (hNAME) increased the magnitude of PEinduced contraction. Meanwhile the response to acetylcholine (ACh) was significantly increased in LBP-treated rats, but the response to nitroprusside had no significant difference among groups. Pretreatment with Larginine paltially restored ACh-induced relaxation in RH rats, hut no effect in lsp-treated rats. These results suggest6d that the role of LBP in decreasing vasoconstriction to PE may be mediated by increase of the effects or/and production of endothelium-derived relaxation factor (EDRF). LBP increased formation of EDRF may he related to increase the substrate of EDRF.