Radioimmunoassay of a glycoprotein associated with malignancy |
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| Authors: | Y Hatae T Hatae S D Bolmer M McCarthy E A Davidson |
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| Institution: | Chalmers University of Technology, Department of Engineering Chemistry, S-412 96 Gothenburg Sweden;Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norin Kenkyu Danchi-nai, Ibaraki 305 Japan |
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| Abstract: | A glycoprotein associated with malignancy was purified from the 0.6M perchloric acid-soluble fraction of serum obtained from cancer patients. The purified glycoprotein contained sialic acid, which was responsible for binding to wheat-germ agglutinin-Sepharose. Gel electrophoresis showed one band with an apparent Mr of 50 000-55 000, and the isoelectric point was 4.4 +/- 0.1. The glycoprotein could be distinguished from carcinoembryonic antigen and alpha-fetoprotein. Iodination of this material with chloramine-T permitted development of a radioimmunoassay. |
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