生物转化对二甲苯生成对苯二甲酸的初步研究

对苯二甲酸是生产聚酯的主要原料，其生产方法主要是采用化学合成法。随着生物转化与生物催化研究的深入，其高效、环保、节能等优势越来越明显。筛选能够生物转化对二甲苯生成对苯二甲酸的菌株将会为生物催化法生产对苯二甲酸打下基础。通过建立筛选模型，利用唯一碳源法从土壤中分离筛选得到微生物16，经鉴定为嗜麦芽窄食单胞菌和睾丸酮丛毛单胞菌的混合菌株，该微生物可以利用对二甲苯为底物生物转化生成对苯二甲酸。实验中对诱导剂进行了选择，表明甲苯对该反应有明显的诱导作用，最佳诱导剂加入量为200mg／L。发酵液中对苯二甲酸及中间产物采用高效液相色谱法测定。

Elementary Studies on Biotransformation of p- Xylene into Terephthalic Acid

Terephthalic acid is monomer-having utility in the production of polyesters. A variety of chemical routes to terophthalic acid are known. Biological process to produce terophthalic acid was studied in this thesis. A strain was isolated from the soil samples through a screening procedure. It can oxidize p-xylene to terephthalic acid. Through the experiment, the enzyme activity of 16 was markedly induced by toluene and the yield of terophthalic acid was increased. The optimal concentration of toluene was 200 mg/L The method of high performance liquid chromatography was used for separation and determination of terophthalic acid.