Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. Golden Tower) |
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| Authors: | S. J. Kim S. J. Lee B.-D. Kim K.-H. Paek |
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| Affiliation: | (1) Department of Horticulture, Seoul National University, Suwon 441-744, Korea 2, KR;(2) Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea Fax no.: +82-2-928-1274 E-mail: khpaek95@kuccnx.korea.ac.kr +, KR |
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| Abstract: | We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains. Received: 30 September 1996 / Revision received: 5 May 1997 / Accepted: 22 May 1997 |
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| Keywords: | Cucumber mosaic virus Local lesion assay Satellite-RNA-mediated protection Transgenic hot pepper plants |
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