Transformation of sunflower (Helianthus annuus L.): a reliable protocol |
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Authors: | Nathalie Knittel Véronique Gruber Günther Hahne Philippe Lénée |
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Institution: | (1) BIOCEM, Campus Universitaire des Cézeaux, 24 av. des Landais, 63170 Aubière, France;(2) Institut de Biologie Moléculaire des Plantes, CNRS et Université Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg, France |
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Abstract: | Summary A reliable protocol for the transformation of cultivated sunflower (Helianthus annuus L.) has been established, based on microprojectile bombardment of half shoot apices in combination with Agrobacterium tumefaciens coculture. Transgenic shoots have been obtained from 5 inbred lines, although transformation efficiencies varied with the genotype. Plants expressing the transgenes could be recovered from up to 7% of the explants. A minority of plants was shown to be chimaeric for expression of ß-glucuronidase activity while most appeared to be uniformly transformed. Genetic segregation was 31 for both ß-glucuronidase and neomycine phospho transferase in some plants, indicating that the respective mother plants were uniformly transformed. Integration of the foreign genes was also shown by Southern analysis.Abbreviations BAP
benzyl amino purine
- EDTA
ethylene diamine tetraacetic acid
- GUS
ß-glucuronidase
- npt II
neomycine phospho-transferase II |
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