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Biotransformation of 14-deacetoxyl sinenxan A by Ginkgo cell suspension cultures and the cytotoxic activity evaluation |
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| Authors: | Yulian Zhan Jian-Hua Zou Xiaojun Ma Jungui Dai |
| Affiliation: | aKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Beijing, PR China bInstitute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050, PR China cInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Beijing 100094, PR China |
| Abstract: | 14-Deacetoxyl sinenxan A [2,5,10β-triacetoxy-4(20),11-taxadiene, 1] was converted to two new products, 10β-hydroxy-2,5-diacetoxy-4(20),11-taxadiene (2) and 10β-butyloxy-2,5-diacetoxy-4(20),11-taxadiene (3) both about in 20% yields by Ginkgo cell suspension cultures. Their structures were identified on the basis of their chemical and spectroscopic data. The three compounds (1–3) were preliminarily evaluated for their in vitro cytotoxic activities against two solid tumor cell lines and their drug-resistant counterparts (KB and KB/V, MCF-7 and MCF-7/ADR), and the decreased activities were observed in the case of the two products. The results suggested that biotransformation might be a valuable approach to diversifying natural products and provide some useful information on the study on the structure–activity relationships of the type of compounds. |
| Keywords: | Biotransformation Taxane Cell suspension cultures Ginkgo biloba L. Cytotoxic activity |
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