| 小麦NBS类抗病基因同源cDNA序列的克隆与特征分析 |
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| 引用本文： | 张楠,王海燕,刘大群.小麦NBS类抗病基因同源cDNA序列的克隆与特征分析[J].植物遗传资源学报,2010,11(5):605-610. |
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| 作者姓名： | 张楠 王海燕 刘大群 |
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| 作者单位： | 河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心,保定,071001 |
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| 基金项目： | 国家自然科学基金(30700505); 河北省自然科学基金(C2008000281); 河北省教育厅(2009130) |
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| 摘 要： | 根据已克隆植物抗病(R)基因NBS保守结构域设计简并引物,采用RT-PCR和cDNA末端快速扩增技术(RACE),在小麦抗叶锈病近等基因系材料TcLr19中进行抗病同源基因cDNA全长的扩增。获得了1个通读的NBS类抗病同源基因S11A11cDNA序列,该序列全长2923bp,编码878个氨基酸序列。生物信息学分析结果表明,该片段含有NB-ARC保守结构域和多个LRR结构域。聚类分析表明,S11A11编码的蛋白与小麦抗叶锈病基因Lr1编码的蛋白亲缘关系较近,而与Lr10亲缘关系较远。半定量RT-PCR分析表明,该基因在小麦叶片中为低丰度组成型表达。本研究在TcLr19小麦中成功获得了抗病基因同源序列,为最终克隆小麦抗叶锈病目的基因奠定了基础。
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| 关 键 词： | 小麦 核苷酸结合位点(NBS) 抗病基因同源序列(RGAs) cDNA末端快速扩增技术(RACE) 半定量RT-PCR |
Cloning and Characterization of a NBS Resistance Gene Homology cDNA Sequence from Wheat |
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| ZHANG Nan,WANG Hai-yan,LIU Da-qun.Cloning and Characterization of a NBS Resistance Gene Homology cDNA Sequence from Wheat[J].Journal of Plant Genetic Resources,2010,11(5):605-610. |
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| Authors: | ZHANG Nan WANG Hai-yan LIU Da-qun |
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| Institution: | ZHANG Nan,WANG Hai-yan,LIU Da-qun(College of Plant Protection,Agricultural University of Hebei/ Biological Control Center ofPlant Disease and Plant Pests of Hebei Province,Baoding 071001) |
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| Abstract: | Resistance gene homology sequence from wheat was isolated by using homology-based method.A pair of degenerated primer was designed according to the nucleotide binding site conserved domains of the cloned plant disease resistance(R) genes.RT-PCR and RACE were used to obtain the full length sequence of the disease resistance homology gene in the near isogenic lines TcLr19.One open-reading NBS class of resistance gene analogs(RGAs) named S11A11 was obtained,which was 2923bp in length and encoded 878 amino acid... |
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| Keywords: | Wheat Nucleotide binding site(NBS) Resistance gene analogs(RGAs) Rapid amplification cDNA end(RACE) Semi-quantitative RT-PCR |
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