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| 高产Monacolin K红曲菌株的复合诱变选育 | |
| 引用本文： | 孙嘉龙,邹晓,刘爱英,梁宗琦,肖唐付.高产Monacolin K红曲菌株的复合诱变选育[J].菌物学报,2007,26(4):507-516. |
| 作者姓名： | 孙嘉龙 邹晓 刘爱英 梁宗琦 肖唐付 |
| 作者单位： | 1. 中国科学院地球化学研究所,环境地球化学国家重点实验室,贵阳,550002;中国科学院研究生院,北京,100039;贵州省环境科学研究设计院,贵阳,550002;贵州省贵州大学真菌资源研究所,花溪,550000 2. 贵州大学真菌资源研究所,花溪,550000 3. 中国科学院地球化学研究所,环境地球化学国家重点实验室,贵阳,550002 |
| 摘 要： | 利用紫外线和氯化锂对一株紫红曲Monascus purpureus菌株进行复合诱变试验。在最佳紫外照射时间为45s和最佳氯化锂浓度为1.0‰的情况下,获得了一株MonacolinK高产突变菌株Monascus purpureus ZT32且连续传接5代产量稳定,用紫外分光光度法检测其MonacolinK的含量为219.9μg/mL,较原始菌株高出2倍多,然后将其用于固态发酵,结果表明:红曲米中的MonacolinK的含量达到8.33mg/g,是原始菌株的3.3倍。 |
| 关 键 词： | 紫红曲 紫外线照射 氯化锂 液体发酵 固态发酵 |
| 文章编号： | 1672-6472(2007)04-0507-0516 |
| 修稿时间： | 2007年4月4日 |
Screening of Monacolin K-high-yielding Monascus strain by combinative mutation treatment |
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| Authors: | SUN Jia-Long ZOU Xiao LIU Ai-Ying LIANG Zong-Qi XIAO Tang-Fu |
| Abstract: | A strain of Monascus purpureus isolated from red yeast rice in Zhangzhou, Fujian Province, was treated with UV irradiation, and then the spore suspension was incubated in media containing LiCl. The exposure time under UV is 45s and the concentration of LiCl in the medium is 1.0‰.The mutant Monascus purpureus ZT32 was obtained and detected by ultraviolet spectrophotometry. The content of Monacolin K for the fifth subsequent generation of subcultures reached 219.9μg/mL, more two times than that for the original strain. In the solid-state fermentation of red yeast rice using the mutant, the production of Monacolin K attained 8.33mg/g, 3 times than that of the original strain. |
| Keywords: | Monascus purprueus UV irradiation LiCl liquid fermentation solid-state fermentation |
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