Construction and genetic characterization of lambda specialized transducing particles carrying therglB gene ofEscherichia coli K-12 |
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| Authors: | Shanmugasundaram Sozhamannan Dr Kuppamuthu Dharmalingam |
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| Institution: | (1) Department of Biotechnology, School of Biological Sciences, Madurai Kamaraj University, 625 021 Madurai, India |
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| Abstract: | TherglB gene ofEscherichia coli codes for a restriction activity that cleaves the hydroxymethylated DNA of T2 and T4 phages. Earlier mapping data placed the gene at 98.39 min counterclockwise to thehsd operon. Genetic analysis of the in vivo gene fusions with  fusion-transducing phages established the location of therglB gene next to thehsdS gene of thehsdRMS cluster. The methodology used in this study could be extended to similar in vivo physical mapping of closely linked genes. |
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