Enhancement of plasmid stability and enzymatic expression by immobilising recombinant Saccharomyces cerevisiae |
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| Authors: | T. Lú Chau A. Guillán E. Roca M.J. Núñez J.M. Lema |
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| Affiliation: | (1) Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Av. das Ciencias s/n, 15706 Santiago de Compostela, Spain |
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| Abstract: | Immobilisation of cells in a calcium alginate gel improved plasmid stability (up to 50%) and enzymatic expression (up to 57%) of a recombinant Saccharomyces cerevisiaeover-expressing the homologous gene EXG1. The rate of segregational loss in the free cells was 14-fold higher than that of the immobilised cells. Recombinant protein synthesis requires reduced cofactors, which affect the redox balance of the cell. |
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| Keywords: | enzymatic expression immobilisation plasmid stability recombinant S. cerevisiae |
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