Cytotoxic and apoptosis-inducing activities against human lung cancer cell lines of cassaine diterpenoids from the bark of Erythrophleum fordii |
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| Authors: | Manh Tuan Ha Manh Hung Tran Thien Thuong Phuong Jeong Ah Kim Mi Hee Woo Jae Sue Choi Suhyun Lee Jeong Hyung Lee Hyeong Kyu Lee Byung Sun Min |
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| Institution: | 1. College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea;2. Department of Bioactive Products, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay District, Hanoi, Viet Nam;3. Biomedical Science Department, VNUK Institute for Research and Executive Education, The University of Danang, 158A Le Loi, Hai Chau District, Danang City, Viet Nam;4. National Institute of Medicinal Materials, 3B Quang Trung Street, Hanoi, Viet Nam;5. College of Pharmacy, Kyungpook National University, Daegu 702-701, Republic of Korea;6. Faculty of Food Science and Biotechnology, Pukyung National University, Busan 608-737, Republic of Korea;7. College of Natural Science, Kangwon National University, Kangwon 200-701, Republic of Korea;8. Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, Chungbuk 363-883, Republic of Korea |
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| Abstract: | A phytochemical investigation into the bark of Erythrophleum fordii yielded four new compounds, two new cassaine diterpenoids (erythrofordin T and U, 1 and 2) and two new cassaine diterpenoid amines (erythroformine A and B, 6 and 7), as well as nine known compounds. We report for the first time the isolation of erythrofordin V (3) from a natural source and that of the remaining eight known diterpenoids (4–5, 8–13) from E. fordii. All structures were elucidated using spectroscopic analysis. Cytotoxic activity of the isolated compounds (1–13) was examined in vitro against three non-small cell lung cancer cell lines (A549, NCI-H1975, and NCI-H1229) using the MTT assay. Cassaine diterpene amines (6–10, 12, 13) exhibited potent cytotoxic activity against all three cell lines with IC50 values between 0.4 μM and 5.9 μM. Erythroformine B (7) significantly induced apoptosis in all three cancer cells in a concentration-dependent manner. |
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| Keywords: | Cassaine diterpene alkaloid Non-small cell lung cancers (NSCLC) Apoptosis |
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