Adh
1
first intron polymorphisms in Argentinean populations of Ceratitis capitata |
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Authors: | SB Lanzavecchia MI Remis JL Cladera RO Zandomeni |
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Institution: | 1. Laboratorio de Genética de Insectos de Importancia Económica, Instituto de Genética ‘Ewald A. Favret’, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), Castelar, Buenos Aires, Argentina;2. Laboratorio de Genética de Poblaciones Aplicada, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina;3. Laboratorio de Alta Complejidad, Instituto de Microbiología y Zoología Agrícola, CICVyA, INTA, Castelar, Buenos Aires, Argentina |
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Abstract: | DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing‐polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three‐allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy–Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations from two African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. |
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Keywords: | population genetics exon priming intron crossing‐polymerase chain reaction medfly molecular markers migration patterns gene flow Diptera Tephritidae EPIC‐PCR |
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