Primary cultures of rat pancreatic acinar cells in serum-free medium |
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| Authors: | Patsy M. Brannon Bonnie M. Orrison Norman Kretchmer |
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| Affiliation: | (1) Laboratory of Developmental Gastroenterology and Nutrition Neonatal and Pediatric Medicine Branch, National Institute of Child Health and Human Development, National Institutes of Health, 20205 Bethesda, Maryland;(2) Present address: Dept. of Nutrition and Food Science, University of Arizona, 309 Agricultural Sciences Bidg., 85721 Tucson, AZ;(3) Present address: Pregnaney and Reproduction Branch, NICHD, NIH, 20205 Bethesda, MD;(4) Present address: Dept. of Nutritional Sciences, University of California-Berkeley, 308 Morgan Hall, 94720 Berkeley, CA |
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| Abstract: | Summary Rat pancreatic acinar cells were isolated and cultured in Ham's F12 medium with 15% bovine calf serum. Caerulein, insulin, somatostatin, and dexamethasone (DEX) had no effect on intracellular or secreted amylase in these cultured cells. A serum-free medium, using Waymouth's MB 752/1 supplemented with albumin, epidermal growth factor (EGF), DEX, and HEPES, was then developed to avoid serum factors that might mask hormonal effects. In this SF medium, pancreatic acinar, cells maintained the morphological and ultrastructural characteristics of freshly isolated cells and secreted amylase in response to the secretagogue, carbamyl choline. Insulin, at a concentration of 1 μg/ml, significantly increased intracellular and secreted amylase activity after 3 d. This model cell system can be used to study the regulation of the synthesis of amylase and other pancreatic enzymes in vitro. |
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| Keywords: | amylase dietary adaptation insulin exocrine pancreas |
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